Single molecule spectroscopy (SMS) analysis suite

Full SMS is a comprehensive graphical user interface (GUI)-driven software package for processing and analysing multi parameter SMS datasets. These datasets typically include fluorescence intensity and lifetime, as well as spectral properties of single dye molecules, quantum dots, or fluorescent active biomolecules.

The software enables detailed analysis through tools for statistical evaluation of fluorescence intensity, clustering, and level identification; lifetime decay fitting; and second-order correlation function calculations. Full SMS is used to analyse three sample types as case studies to showcase its utility and ease-of-use. Complementing the software, this study also presents a custom-designed sample cell holder, which extends the capabilities of the SMS experimental setup designed and assembled in-house.