Table · dataset · 2026
<b>Two-Component System TCS1 Contributes to Heat and Acid Stress Tolerance and Virulence in </b><b><i>Streptococcus pasteurianus</i></b>
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<p dir="ltr"><a href="" target="_blank"><i>Streptococcus pasteurianus</i> is an emerging zoonotic pathogen associated with meningitis and bacteremia in both animals and humans, yet the molecular mechanisms underlying its pathogenicity remain poorly understood.
Description
In this study, comparative genomic analysis of virulent strain WUSP067 and non-virulent strain WUSP074 identified 280 genes unique to WUSP067, including six candidate virulence genes.</a> Among these, four genes encoding putative transcriptional regulatory proteins were selected for further characterization.
Homology analysis and conserved domain prediction indicated that these genes constitute two distinct two-component systems (TCSs), designated TCS1 and TCS2. Both systems shared 26–36% amino acid identity with TCS01, a regulatory system previously implicated in antimicrobial peptide resistance in <i>Streptococcus pneumoniae</i>. Antimicrobial susceptibility testing demonstrated that neither TCS1 nor TCS2 altered the resistance profile of <i>S. pasteurianus</i> against the tested antibacterial agents.
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In contrast, deletion of <i>tcs1</i> significantly impaired bacterial survival in porcine whole blood and attenuated virulence in a mouse infection model, indicating its role in pathogenicity. Transcriptomic and RT-qPCR analyses further showed that TCS1 regulates the expression of heat shock protein (HSP) associated genes, including the <i>groES-groEL</i> operon, the <i>hrcA-grpE-dnaJ-dnaK</i> operon, and <i>clpE</i>. Consistently, the Δ<i>tcs1</i> mutant showed impaired growth under heat stress (43 ℃) and acidic conditions (pH 4.0).
Electrophoretic mobility shift assays further suggested that the response regulator of TCS1 indirectly modulates the expression of these HSP related genes. Together, these findings demonstrate that TCS1 contributes to <i>S. pasteurianus</i> virulence by promoting adaptation to heat and acid stresses encountered in host environments.</p>
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- DOI doi.org/10.6084/m9.figshare.33719329.v1 ↗
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Provenance · 1 source records, 17 field assertions
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