Data · study · 2026
Molecular characterization of the uterotrophic assay: Temporal gene expression changes in uterus and pituitary gland following ethinylestradiol
Listed in NCBI GEO
an OECD-compliant uterotrophic assay was performed using ethinyl estradiol (EE) in juvenile female rats, followed by an analysis of the temporal gene expression profiles and associated pathways in uterus and pituitary gland in comparison to the standard parameters like uterine weight and histopathology.
Description
Additionally, published gene and protein expression analyses of uteri were included for evaluation of overall common pathways.
Immature, 15-day old female Wistar rats (strain Hsd/Cpb:WU; Harlan) were maintained under controlled conditions of temperature, relative humidity, and illumination. Animals had free access rat pellet diet and drinking water ad libitum. At the age of 19 days, rats were randomly assigned to groups (n=3-6) and were subcutaneously dosed with either 3 µg/kg ethinyl estradiol (EE, Sigma) or control (corn oil,) at 4 ml/kg body weight every 24h for three consecutive days.
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The animal experimental procedure was identical to the OECD uterotrophic assay (OECD 2000). Two, four, eight, and 24h after the first, four and 24h after the second, and 24h after the last application, animals were sacrificed in deep anesthesia (see Fig.1A) and uteri, pituitary glands and vaginas were rapidly removed. Uterine wet weight was determined, then uteri were longitudinally cut in halves.
The right uterine halves and the pituitary glands for RNA isolation were shock frosted with liquid nitrogen and stored at −80°C.
Links
Get the data
- GEO FTP directory ftp.ncbi.nlm.nih.gov/geo/series/GSE338nnn/GSE338902 ↗
download · from NCBI GEO
Where it is published
- GEO accession page ncbi.nlm.nih.gov/geo/query/acc.cgi?acc=GSE338902 ↗
landing page · from NCBI GEO
Documentation and papers
- PRJNA1496347 ncbi.nlm.nih.gov/bioproject/PRJNA1496347 ↗
project · from NCBI GEO
- PubMed 42474487 pubmed.ncbi.nlm.nih.gov/42474487 ↗
publication · from NCBI GEO
Topics
- Stated by source
- Expression profiling by array · Rattus norvegicus
- From keywords
- Life Sciences
Provenance · 1 source records, 7 field assertions
| Source | Key | Last seen | Raw |
|---|---|---|---|
| NCBI GEO | GSE338902 | 13 d ago | JSON v1 |
| Field | Assertion | Extractor | Evidence |
|---|---|---|---|
| access_level | source · NCBI GEO | connector:ncbi_geo@1.0.0 | |
| concepts[field].local:field:life-sciences | mapping · NCBI GEO | connector:ncbi_geo@1.0.0 | |
| concepts[method].geo_series_type:expression-profiling-by-array | source · NCBI GEO | connector:ncbi_geo@1.0.0 | /gdstype |
| concepts[organism].NCBITaxon:10116 | source · NCBI GEO | connector:ncbi_geo@1.0.0 | /taxon |
| description | source · NCBI GEO | connector:ncbi_geo@1.0.0 | /summary |
| publication_date | source · NCBI GEO | connector:ncbi_geo@1.0.0 | |
| title | source · NCBI GEO | connector:ncbi_geo@1.0.0 | /title |