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Data · dataset · 2019

RISC Kinetic and Thermodynamic Data

Listed in Teesside University Research Data Repository

We applied high-throughput methods to measure the association kinetics, equilibrium binding energies, and single- turnover cleavage rates of RISC.

Description

We find that RISC readily tolerates insertions of up to seven nucleotides in its target opposite the central region of the guide. Fit parameters for miR-21 and let-7a association, equilibrium binding, and cleavage are provided herein.

These data were obtained from RNA-MaP and RISC Cleave-'n-Seq. Raw sequencing data is available on SRA.

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Where it is published

Catalogue records · 1

Topics

From keywords
Earth & Environmental Science
Inferred from text
Sequencing 75%
Provenance · 1 source records, 7 field assertions
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Teesside University Research Data Repositoryoai:data.mendeley.com/fzh7pfpmmb.114 h agoJSON v1
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concepts[modality].local:modality:sequencingenrichment · researchdata tees ac ukkeyword-concept-rules@1.0.0title+description (75%)
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