PHOTOPERIOD 1 enhances stress tolerance and energy metabolism to promote spike fertility in barley under high ambient temperatures

HT delayed inflorescence development and reduced grain number in ppd-h1 mutant lines, while the wild-type Ppd-H1 genotypes exhibited accelerated reproductive development and showed a stable grain set under HT. Using a CRISPR/Cas9-induced ppd-h1 mutant, we confirmed that the CCT domain of Ppd-H1 controls developmental stability, but not clock gene expression. Transcriptome and phytohormone analyses revealed increased stress-responsive gene expression and abscisic acid levels in the leaf and inflorescence of the natural and induced mutant ppd-h1 lines.

Furthermore, the ppd-h1 lines displayed downregulated photosynthesis- and energy metabolism-related genes, as well as decreased auxin and cytokinin levels in the inflorescence, which impaired anther and pollen development. In contrast, the transcriptome, phytohormone levels, and anther and pollen development remained stable under HT in the wild-type Ppd-H1 plants. Our findings suggest that Ppd-H1 enhances stress tolerance and energy metabolism, thereby stabilizing reproductive development, floret fertility and grain set under HT.