Data · dataset · 2023
The mass spectrometry of purified lipovitellin sample from zebrafish embryo lysate
Listed in ScienceDB
Our preliminary research found that lipovitellin (LV) is an abundant DNA-binding protein in early zebrafish embryos.
Description
LV is the main component of vitellogenin and the largest protein of vitellogenin related products. LV is traditionally regarded as an energy reserve for developing embryos and has been shown to be involved in immune defense of developing embryos in fish.
Here, purified Lv was obtained from 0 h zebrafish embryo lysate using Sephadex G-200 column combined with QAE-Sephadex A50 anion exchange column. The purified protein sample was analyzed with Easy-nLC 1200 high performance liquid chromatography (Shanghai Luming Biotechnology Co., Ltd., China). We found that although the results of mass spectrometry showed the presence of 69 proteins in the sample, LV accounted for 99.22 % of them.
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Table 1 shows all the protein types detected by mass spectrometry.
Links
Where it is published
- DOI doi.org/10.57760/sciencedb.12153 ↗
DOI / persistent id · from scidb cn
Catalogue records · 1
- OAI-PMH record scidb.cn/oai?verb=GetRecord&metadataPrefix=oai_dc&identifier=10.57760%2… ↗
metadata API · from scidb cn
Topics
- From keywords
- Earth & Environmental Science · Engineering · Humanities · Life Sciences · Social Science
- Inferred from text
- Mass spectrometry 75% · Tabular 65%
Provenance · 1 source records, 12 field assertions
| Source | Key | Last seen | Raw |
|---|---|---|---|
| ScienceDB | 10.57760/sciencedb.12153 | 8 d ago | JSON v1 |
| Field | Assertion | Extractor | Evidence |
|---|---|---|---|
| access_level | source · scidb cn | connector:scidb_cn@1.0.0 | |
| concepts[field].local:field:earth-environmental | mapping · scidb cn | connector:scidb_cn@1.0.0 | |
| concepts[field].local:field:engineering | mapping · scidb cn | connector:scidb_cn@1.0.0 | |
| concepts[field].local:field:humanities | mapping · scidb cn | connector:scidb_cn@1.0.0 | |
| concepts[field].local:field:life-sciences | mapping · scidb cn | connector:scidb_cn@1.0.0 | |
| concepts[field].local:field:social-science | mapping · scidb cn | connector:scidb_cn@1.0.0 | |
| concepts[modality].local:modality:mass-spectrometry | enrichment · scidb cn | keyword-concept-rules@1.0.0 | title+description (75%) |
| concepts[modality].local:modality:tabular | enrichment · scidb cn | keyword-concept-rules@1.0.0 | title+description (65%) |
| description | source · scidb cn | connector:scidb_cn@1.0.0 | /metadata/dc/description |
| license | source · scidb cn | connector:scidb_cn@1.0.0 | /metadata/dc/rights |
| publication_date | source · scidb cn | connector:scidb_cn@1.0.0 | |
| title | source · scidb cn | connector:scidb_cn@1.0.0 | /metadata/dc/title |