Experimental and coastscape data on salinity responses and potential hyposaline exposure in Anomalocardia flexuosa

Binary values indicate whether the valves were observed open or closed. valve_24hrs.csv - Individual valve-opening observations recorded after 24 h of exposure to nine experimental salinities. Missing values indicate individuals that had burrowed and whose valve position could not be observed. valve_28hrs.csv - Individual valve-opening observations recorded after 28 h of exposure to nine experimental salinities. The observation followed the repositioning of specimens with the umbo facing upward. osm.csv - Individual hemolymph osmolality measurements obtained after 28 h of exposure to nine experimental salinities.

Osmolality is expressed in mOsm kg⁻¹. th.csv - Individual tissue hydration measurements obtained after 28 h of exposure to nine experimental salinities. Tissue hydration is expressed as the percentage of water relative to tissue wet mass. lactato.csv - Individual lactate concentrations measured in adductor-muscle samples after 28 h of exposure to nine experimental salinities. ldh.csv - Individual lactate dehydrogenase activity measurements obtained from adductor-muscle samples after 28 h of exposure to nine experimental salinities. prot.csv - Total protein concentrations measured in adductor-muscle homogenates and used to normalize lactate dehydrogenase activity.

Bio-ORACLE v3 sea-surface salinity data used to characterize background salinity conditions across the environmental units. WorldClim 2.1 bioclimatic data used to quantify annual precipitation, wettest-quarter precipitation, and precipitation seasonality. HydroRIVERS data and derived river-mouth locations used to quantify river discharge, drainage characteristics, and the distance between occurrence areas and river mouths.