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Data · dataset · 2026

<p>Additional biochemical characterization of the ORC4R protein.</p>

Listed in ZivaHub and Deakin Research Online and DMU Figshare — shown once because both records carry DOI 10.1371/journal.pone.0349465.s005

<p>A) Comparison of DNA binding of WT ORC, ORC4R with and without the Orc4-<i>sofa</i> mutation, and ORC1K in electrophoretic-mobility shift assay (EMSA).

Description

Binding in the presence of ATP or ATPγS is shown. B) Sequence specificity of ORC and ORC4R DNA binding.

EMSA was used to measure wild-type ORC and ORC4R DNA binding to WT <i>ARS1</i> or <i>ARS1</i> lacking the strongest ORC binding site (A-). C) Titration of WT ORC, ORC4R, and ORC1K proteins in a helicase-loading assay (0.3 mM ATP). D) Salt titration and sequence-specific loading of the WT and ORC4R protein (R267A).

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Sequence specificity is tested using a WT <i>ARS1</i> and an <i>ARS1</i> lacking the two strongest ORC binding sites (A-/B2-).</p> <p>(TIF)</p>

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Catalogue records · 1

Topics

From keywords
Biochemistry and cell biology · Biochemistry and cell biology · Biochemistry and cell biology · Earth & Environmental Science · Earth & Environmental Science · Earth & Environmental Science · Genetics · Genetics · Genetics · Life Sciences · Life Sciences · Life Sciences · Saccharomyces cerevisiae · Saccharomyces cerevisiae · Saccharomyces cerevisiae
Provenance · 3 source records, 20 field assertions
SourceKeyLast seenRaw
ZivaHuboai:figshare.com:article/3403555510 d agoJSON v1
Deakin Research Onlineoai:figshare.com:article/3403555510 d agoJSON v1
DMU Figshareoai:figshare.com:article/3403555510 d agoJSON v1
FieldAssertionExtractorEvidence
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concepts[field].anzsrc:group:3101mapping · figshare dmu ac ukvocabulary-mapper@1.0.0keywords['Cell Biology']
concepts[field].anzsrc:group:3101mapping · dro deakin edu auvocabulary-mapper@1.0.0keywords['Cell Biology']
concepts[field].anzsrc:group:3101mapping · zivahub uct ac zavocabulary-mapper@1.0.0keywords['Cell Biology']
concepts[field].anzsrc:group:3105mapping · dro deakin edu auvocabulary-mapper@1.0.0keywords['Genetics']
concepts[field].anzsrc:group:3105mapping · zivahub uct ac zavocabulary-mapper@1.0.0keywords['Genetics']
concepts[field].anzsrc:group:3105mapping · figshare dmu ac ukvocabulary-mapper@1.0.0keywords['Genetics']
concepts[field].local:field:earth-environmentalmapping · figshare dmu ac ukconnector:figshare_dmu_ac_uk@1.0.0
concepts[field].local:field:earth-environmentalmapping · dro deakin edu auconnector:dro_deakin_edu_au@1.0.0
concepts[field].local:field:earth-environmentalmapping · zivahub uct ac zaconnector:zivahub_uct_ac_za@1.0.0
concepts[field].local:field:life-sciencesmapping · figshare dmu ac ukconnector:figshare_dmu_ac_uk@1.0.0
concepts[field].local:field:life-sciencesmapping · dro deakin edu auconnector:dro_deakin_edu_au@1.0.0
concepts[field].local:field:life-sciencesmapping · zivahub uct ac zaconnector:zivahub_uct_ac_za@1.0.0
concepts[organism].NCBITaxon:4932mapping · figshare dmu ac ukvocabulary-mapper@1.0.0keywords['saccharomyces cerevisiae </']
concepts[organism].NCBITaxon:4932mapping · zivahub uct ac zavocabulary-mapper@1.0.0keywords['saccharomyces cerevisiae </']
concepts[organism].NCBITaxon:4932mapping · dro deakin edu auvocabulary-mapper@1.0.0keywords['saccharomyces cerevisiae </']
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titlesource · zivahub uct ac zaconnector:zivahub_uct_ac_za@1.0.0/metadata/dc/title