Transcriptional profiling of cecum from mice flown on the RR-10 mission

Following removal of hindlimbs, carcasses were wrapped in aluminum foil, preserved in the CryoChiller, and stored at -80 C or colder until return to Earth. In addition to the Flight group, three ground control groups were also part of the study: Basal (representing the pre-launch state), Vivarium (standard vivarium housing for the same duration of time as flight), and Ground (flight habitat in the International Space Station Environment Simulator, ISSES).

Twenty mice (10 of each strain) were included in each of these control groups (except Vivarium which included 12 of each strain). These were treated, euthanized and processed on the same schedule and in the same manner as the flight samples. This study includes bulk RNA sequencing ribodepleted gene expression data from the cecum of 20 Basal animals (10 WT and 10 p21-null), 10 Flight animals (5 WT and 5 p21-null), 10 Ground animals (5 WT and 5 p21-null), and 24 Vivarium animals (12 WT and 12 p21-null).