Supplementary file 1_Exogenous 24-epibrassinolide enhances chilling tolerance in Phalaenopsis-type Dendrobium through coordinated physiological and transcriptomic responses.docx

Introduction<p>Phalaenopsis-type Dendrobium is a commercially important tropical orchid that is highly sensitive to chilling, which can cause premature leaf senescence and abscission.</p>Methods<p>We evaluated the protective effect of exogenous 24-epibrassinolide (EBR) and integrated time-resolved RNA-seq with physiological traits previously characterized in the same experimental framework. A concentration of 2.0 mg/L was selected for transcriptomic analysis based on the refined dose-response experiment.

WGCNA was performed using 18 EBR-treated libraries. Global GO and KEGG outputs were retained as descriptive overviews, whereas quantitative functional inference was based on module-specific analyses with complete multiple-testing-adjusted statistics.</p>Results<p>EBR delayed leaf yellowing and abscission. Differential-expression analysis revealed time-dependent transcriptional responses during chilling and recovery.