Data · dataset · 2026
<p>Vacuolar fission precedes HTC accumulation in <i>Pf</i>ATG18-deficient parasites.</p>
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<p>Synchronized parasites were treated with DMSO or RAP from the ring stage onward and imaged at 28 and 36 hours post-invasion (hpi). <b>(A-C)</b> Early DV fragments in <i>Pf</i>ATG18-deficient parasites still receive cargo.
Description
E64-mediated inhibition of hemoglobin degradation causes bloating in both intact and fragmented DVs. Representative images of CRT-mNG (A) and Giemsa-stained blood smears <b>(B)</b>.
White outlines: parasite and host cell; yellow arrowheads: bloated DV (fragments) containing host cell cytosol and hemozoin. Bloating was quantified using Giemsa-stained blood smears <b>(C)</b>. Individual and mean values; paired <i>t</i>-test; n = 5. **, <i>p</i> < 0.01. <b>(C)</b> In <i>Pf</i>ATG18-deficient parasites, the PI3P-marker P40PX-mSc remains restricted to the DVM at the onset of fragmentation and only later appears on accumulating HTCs.
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Yellow arrowheads: P40PX-mSc-positive HTCs lacking CRT-mNG labelling. Vesicular compartments are visible in DIC at 36 hpi, but not at 28 hpi. Scale bars: 5 µm.
Source data are provided as a <a href="plospathogens.org/article/info:doi/10.1371/journal.ppat.1014608#ppat.1014608.s015" target="_blank">S3 File</a>.</p>
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Where it is published
- DOI doi.org/10.1371/journal.ppat.1014608.g004 ↗
DOI / persistent id · from zivahub uct ac za
Catalogue records · 1
- OAI-PMH record api.figshare.com/v2/oai?verb=GetRecord&metadataPrefix=oai_dc&identifier=oai%3Af… ↗
metadata API · from zivahub uct ac za
Topics
- From keywords
- Biochemistry and cell biology · Earth & Environmental Science · Genetics · Infectious diseases · Life Sciences · Medicine & Health · Microbiology
- Inferred from text
- Image 65%
Provenance · 1 source records, 13 field assertions
| Source | Key | Last seen | Raw |
|---|---|---|---|
| ZivaHub | oai:figshare.com:article/34017857 | 11 d ago | JSON v1 |
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| concepts[field].anzsrc:group:3101 | mapping · zivahub uct ac za | vocabulary-mapper@1.0.0 | keywords['Cell Biology'] |
| concepts[field].anzsrc:group:3105 | mapping · zivahub uct ac za | vocabulary-mapper@1.0.0 | keywords['Genetics'] |
| concepts[field].anzsrc:group:3107 | mapping · zivahub uct ac za | vocabulary-mapper@1.0.0 | keywords['Microbiology'] |
| concepts[field].local:field:earth-environmental | mapping · zivahub uct ac za | connector:zivahub_uct_ac_za@1.0.0 | |
| concepts[field].local:field:life-sciences | mapping · zivahub uct ac za | connector:zivahub_uct_ac_za@1.0.0 | |
| concepts[field].local:field:medicine-health | mapping · zivahub uct ac za | connector:zivahub_uct_ac_za@1.0.0 | |
| concepts[modality].local:modality:image | enrichment · zivahub uct ac za | keyword-concept-rules@1.0.0 | title+description (65%) |
| description | source · zivahub uct ac za | connector:zivahub_uct_ac_za@1.0.0 | /metadata/dc/description |
| license | source · zivahub uct ac za | connector:zivahub_uct_ac_za@1.0.0 | /metadata/dc/rights |
| publication_date | source · zivahub uct ac za | connector:zivahub_uct_ac_za@1.0.0 | |
| title | source · zivahub uct ac za | connector:zivahub_uct_ac_za@1.0.0 | /metadata/dc/title |