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Data · dataset · 2015

Cloning and tissue expression profiling of the GnRHR gene of the Muscovy duck ( Cairina moschata ): Polymorphism and association with egg-laying performance

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The cDNA sequence of the GnRHR gene was cloned and an association analysis between mutations and laying performance was conducted.2. A 1680-bp cDNA sequence of Muscovy duck GnRHR , which encodes 415 amino acids, was obtained and characterised.

Phylogenetic analysis revealed that Muscovy duck GnRHR has a close relationship with Gallus gallus GnRHR .3. There were significantly different expression profiles between 4 age periods in the hypothalamus, pituitary, and ovary. The expression of GnRHR at the age of 36 weeks (laying period) was higher than other time points in the three tissues.

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GnRHR was expressed in 12 different tissues. The highest expression levels were observed in the hypothalamus, pituitary and gonads.4. A single nucleotide polymorphism detected in the second intron was associated with egg-laying performance.

Individuals with the genotype TT had better egg-laying performance from individuals with the genotypes CC or TC. Therefore, GnRHR could be used as a marker gene for laying performance in the Muscovy duck.

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Chemistry · Life Sciences

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DataCite10.6084/m9.figshare.1284350.v112 d agoJSON v1
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concepts[field].local:field:chemistrymapping · DataCitevocabulary-mapper@1.0.0keywords['Chemistry']
concepts[field].local:field:life-sciencesmapping · DataCitevocabulary-mapper@1.0.0keywords['Biological Sciences']
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