Excel · study · 2026
The transcription factor MtCLR-2 regulates cellulase production by directly modulating the expression of Mtegl2 and Mtbgl1 in Myceliophthora thermophila
Listed in NCBI GEO
The thermophilic fungus Myceliophthora thermophila is capable of secreting large amounts of lignocellulolytic enzymes, such as cellulases and xylanases, which are regulated by multiple transcription factors.
Description
However, the understanding of the regulatory mechanism of cellulase gene expression in M. thermophila is limited. Here, we characterized the function of MtCLR-2, a M. thermophila ortholog of CLR-2, a key cellulolytic transcriptional regulator initially identified in Neurospora crassa.
Deletion of Mtclr-2 significantly reduced cellulase activities, particularly affecting endoglucanase production, whereas overexpression of Mtclr-2 led to marked elevation in cellulase secretion when M. thermophila was grown on Avicel. Subcellular localization assay of MtCLR-2 fused to green fluorescent protein (GFP) indicated that MtCLR-2 is localized to the nucleus. Real-time quantitative reverse transcription PCR (RT-qPCR) analysis revealed that disruption of Mtclr-2 caused a decrease in transcript levels of the β-glucosidase gene bgl1 (MYCTH_66804) and the endoglucanase gene egl2 (MYCTH_86753) throughout the stages of growth in cellulose medium.
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Furthermore, electrophoretic mobility shift assays (EMSAs) demonstrated that MtCLR-2 directly binds to the promoter regions of bgl1 and egl2 in a zinc-dependent manner. The comparative transcriptomic analysis also showed that MtCLR-2 positively regulates the expression of ribosomal protein genes under cellulosic conditions. These findings contribute to a better understanding of the regulatory network governing cellulase gene expression and provide a potential target for boosting cellulase biosynthesis in M. thermophila.
Links
Get the data
- GEO FTP directory ftp.ncbi.nlm.nih.gov/geo/series/GSE305nnn/GSE305312 ↗
download · from NCBI GEO
Where it is published
- GEO accession page ncbi.nlm.nih.gov/geo/query/acc.cgi?acc=GSE305312 ↗
landing page · from NCBI GEO
Documentation and papers
- PRJNA1305229 ncbi.nlm.nih.gov/bioproject/PRJNA1305229 ↗
project · from NCBI GEO
- PubMed 42046157 pubmed.ncbi.nlm.nih.gov/42046157 ↗
publication · from NCBI GEO
Topics
- Stated by source
- Expression profiling by high throughput sequencing
- From keywords
- Life Sciences
Provenance · 1 source records, 6 field assertions
| Source | Key | Last seen | Raw |
|---|---|---|---|
| NCBI GEO | GSE305312 | 12 d ago | JSON v1 |
| Field | Assertion | Extractor | Evidence |
|---|---|---|---|
| access_level | source · NCBI GEO | connector:ncbi_geo@1.0.0 | |
| concepts[field].local:field:life-sciences | mapping · NCBI GEO | connector:ncbi_geo@1.0.0 | |
| concepts[method].geo_series_type:expression-profiling-by-high-throughput-sequencing | source · NCBI GEO | connector:ncbi_geo@1.0.0 | /gdstype |
| description | source · NCBI GEO | connector:ncbi_geo@1.0.0 | /summary |
| publication_date | source · NCBI GEO | connector:ncbi_geo@1.0.0 | |
| title | source · NCBI GEO | connector:ncbi_geo@1.0.0 | /title |