A Novel Attenuated and Marker M. bovis-BoHV-1 Combined Vaccine Provides Broad Protection Against Diverse Bovine Herpesvirus Type 1 Genotypes

Both the combined vaccine group and the inactivated vaccine group had significantly lower titers and shorter duration of virus shedding compared to the non-immune challenge group, for both BoHV-1 strain challenges. Specifically, the combined vaccine group had about 2 titers reduction in peak virus titers and a 6-8 day earlier clearance of virus shedding compared to the non-immune challenge group.

Notably, the combined vaccine group demonstrated slightly better performance in limiting virus shedding of the different BoHV-1 subtypes compared to the inactivated vaccine group, indicating the combined vaccine may provide better cross-protection. These findings highlight the protective efficacy of these vaccines and provide important insights for effective disease control strategies in cattle herds.