Based on network pharmacology and experimental verification, the mechanism of Huangqi Jiaogenin in treating diabetic nephropathy is explored

Establishment of DN mouse model induced by high-fat diet combined with low-dose streptozotocin; By detecting fasting blood glucose, renal function related indicators, evaluating renal histopathology, and measuring oxidative stress and inflammatory response related indicators, the renal protective effect of AS-IV is assessed; Network pharmacology screening integrates common targets of AS-IV and DN, and conducts GO and KEGG enrichment analysis to screen key signaling pathways.

Molecular docking of key targets was performed using AutoDock Vina software and validated by Western blot. The results showed that AS-IV can significantly reduce fasting blood glucose, improve renal function, and alleviate renal tissue pathological damage in DN mice; Reduce the levels of tumor necrosis factor - α, interleukin-6, IL-1 β, and malondialdehyde, increase the activity of superoxide dismutase and reduced glutathione, and inhibit the expression of alpha smooth muscle actin in renal tissue.