Supp Figs S1-S10 from Asynchronous Evolution of Epithelium and Stroma Differentiates Precursor Lesions from Pancreatic Cancer

Supplementary Figure S4. A. Hematoxylin and Eosin (H&E) of healthy and PDAC samples profiled with 10x Genomics CytAssist Visium platform, with overlaid Visium spots. Supplementary Figure S5.

A. Dot plot showing marker genes for each spatial domain in spatial transcriptomics data. Supplementary Figure S6. A. Heatmap showing pairwise Pearson correlation between different spatial domains in different tissue types annotated by spatial domain information and tissue type.

Supplementary Figure S7. A. Heatmap showing scaled expression of leading edge genes from GSEA of OXIDATIVE_PHOSPHORYLATION, FATTY_ACID_METABOLISM and BILE_ACID_METABOLISM. Supplementary Figure S8.

A. Heatmap showing scaled expression of leading edge genes from GSEA of TNFA_SIGNALING_VIA_NFKB, EPITHELIAL_MESENCHYMAL_TRANSITION, INTERFERON_ ALPHA_RESPONSE, HYPOXIA, KRAS_SIGANLING_UP and IL6_JAK_STAT3_SIGNALING. Supplementary Figure S9. A. Spatial map showing the distribution of plasma cells and macrophages around neoplastic domains (PanIN, GLTumor and PDTumor) in healthy, adjacent normal and PDAC samples.

Supplementary Figure S10. A. Spatial map showing the abundance and distribution of tertiary lymphoid structures around neoplastic domains in healthy, adjacent normal and PDAC tissue samples. Colors represent annotated spatial domains.</p>