Functional dissection of Wag31 domains for septal recruitment and polar distribution during the cell cycle

SUPPORTING DATA: Data from the manuscript of Petit et al., "Functional dissection of Wag31 domains for septal recruitment and polar distribution during the cell cycle”. - Alignments/ - Wag31_Bacteria.aln DivIVA/Wag31 multiple sequence alignment in all Bacteria - Wag31_Bacteria_trimmed.aln Trimmed multiple sequence alignment of DivIVA/Wag31 in all Bacteria - Wag31_Bacteria_lineage.tsv Taxonomic lineage annotation for Wag31 protein sequences in Bacteria - Wag31_Actinobacteria_Firmicutes.aln Multiple sequence alignment of Wag31 homologs from representative species of Actinobacteria and Firmicutes. - AlphaFold/ Protein structure predictions of Wag31 dimers from representative species of Actinobacteria and Firmicutes. - Sequencing/ - WT_R1.fq.gz Illumina paired-end reads (R1) of wild-type strain - WT_R2.fq.gz Illumina paired-end reads (R2) of wild-type strain - WT_nanopore.fastq.gz Oxford Nanopore long reads of wild-type strain - MUT_R1.fq.gz Illumina paired-end reads (R1) of the Pino-wag31 conditional mutant - MUT_R2.fq.gz Illumina paired-end reads (R2) of the Pino-wag31 conditional mutant - Assembly/ - Cglut_WT.fasta Hybrid de novo assembly of the parental strain generated with Unicycler - Annotation/ - Cglut_WT.gff3 Bakta annotation of the parental strain assembly - breseq/ breseq report of Pino-wag31 reads mapped against the annotated wild-type assembly