Chromosome-level genome assembly and karyotype validation of the tea tussock moth Euproctis pseudoconspersa

However, nuclear genomic resources suitable for investigating chromosome evolution, population genetics, and host adaptation in this species remain limited. In this study, we integrated PacBio HiFi, Hi-C, and Illumina RNA-sequencing data to generate a chromosome-level reference genome for E. pseudoconspersaand obtained independent cytogenetic evidence through the preparation of mitotic metaphase chromosomes.

The final assembly spanned 356.85 Mb, with contig and scaffold N50 values of 10.22 and 16.80 Mb, respectively. In total, 99.9% of the assembled sequence was anchored to 22 chromosome-scale pseudomolecules. Karyotype analysis revealed a diploid chromosome number of 2n = 44, consistent with the 22 haploid chromosome-scale pseudomolecules supported by the Hi-C data.