CD146 regulates growth factor-induced mTORC2 activity independent of the PI3K and mTORC1 pathways. Xu et al

A separate zip file was created for each figure. The figure panel and a brief description have been labeled for each blots.

For immunoblotting, proteins were resolved in 8-10% SDS-polyacrylamide gel by electrophoresis and then transferred to a polyvinylidene difluoride (PVDF) membrane. The membranes were blocked with 5% non-fat milk in Tris-buffered saline with 0.1% Tween-20 for 1 hour, incubated with primary antibodies at 4℃ overnight and then probed with horseradish peroxidase-conjugated anti-mouse or anti-rabbit secondary antibodies. The signals are visualised using chemiluminescence imaging system (Amersham Imager 600, GE Healthcare Life Sciences).