Genetic and ecological architecture of life cycle transitions in the kelp Laminaria pallida

Variables • replicate: individual ID for each petri dish • strain: strain or cross identifier • mate: absence or presence of mate of the opposite sex in the medium • day: timepoint of the measurement • fields: fields of view counted • area_cm2: area conted based on fields of view • total_gam: absolute number of gametophytes counted • gam_cm2: density of gametophytes / cm2 • m_veg: number of vegetative male gametophytes • m_mat: number of reproductive male gametophytes • m_tra: number of male transition phenotypes • m_pSP_abs: number of male parthenosporophytes • f_veg: number of vegetative female gametophytes • f_mat: number of reproductive female gametophytes • f_egg: number of female gametophytes with released eggs • f_pSP_abs: number of female parthenosporophytes • all_pSP_abs: number of all parthenosporophytes • eggs_abs: absolute number of released eggs File: Timecourse_counts.xlsx Description: Developmental experiment on Laminaria pallida gametophytes to quantify parthenogenetic reproduction.

Variables • replicate: individual ID for each petri dish • strain: strain or cross identifier • setup: single strain or cross of male and female • day: timepoint of the measurement • fields: fields of view counted • area_cm2: area conted based on fields of view • total_gam: absolute number of gametophytes counted • gam_cm2: density of gametophytes / cm2 • m_veg: number of vegetative male gametophytes • m_mat: number of reproductive male gametophytes • m_tra: number of male transition phenotypes • f_veg: number of vegetative female gametophytes • f_mat: number of reproductive female gametophytes • f_egg: number of female gametophytes with released eggs • f_sporo: number of female gametophtes with attached sporophytes • sporo_abs: absolute number of (partheno-)sporophytes File: Microsat_GenAlEx.xlsx Description: Microsatellite genotypes of Laminaria pallida sporophytes collected in Paternoster, South Africa (ZAF) and Swakopmund, Namibia (NAM) in GenAlEx format.

Variables • Individual: individual ID for each sporophyte • Quadrat: Quadrat within each population where sample was taken • CN766672_tri: microsatellite marker with two allele values • Ld_25_penta: microsatellite marker with two allele values • Ld_67_tri: microsatellite marker with two allele values • Lo_15_tri: microsatellite marker with two allele values • Lo_24_tri: microsatellite marker with two allele values • Lo_26_tetra: microsatellite marker with two allele values • Lo_27_tetra: microsatellite marker with two allele values • Lo_28_tetra: microsatellite marker with two allele values • Lo5-8_tri: microsatellite marker with two allele values File: S1_raw_images.tif Description: Annotated raw image file of agarose gel of PCR products with amplified products of sex-specific PCR markers for each Laminaria pallida gametophyte strain and offspring sporophyte lineage used in this study.