Data for: Reducing agents assisted fed-batch fermentation to enhance ABE yields

However, the commercialization of the ABE process has been marred by challenges of low product yield and titer, thereby non-competitive process economics. Here, we coupled low-cost reducing agents with a controlled feeding strategy to improve both product titer and yield.

Reducing agents promote cofactor dependent butanol production while fed-batch operation enhances glucose consumption, final ABE titer, and partly mitigates product toxicity. We investigated the effects of ascorbic acid, L-cysteine, and dithiothreitol (DTT) on fed-batch ABE production using Clostridium acetobutylicum. Moreover, to study the metabolic modifications triggered by these reducing agents, we performed NADH, ATP, extracellular amino acid secretion, and NADH- dependent butanol dehydrogenase (BDH) assays.