Data · dataset · 2026
Identification and functional study of a novel antimicrobial peptide, QUB-2021, from the skin secretion of Odorrana tiannanensis
Listed in ZivaHub and Deakin Research Online and DMU Figshare and UCL Research Data Repository — shown once because both records carry DOI 10.17034/32633337.v1
When a patient is given an antibiotic, there always be a drug resistance.
Description
Moreover, due to the overuse of antibiotics, there has been a rapid increase of antibiotic resistance. Antimicrobial peptides (AMP) found in amphibian skin are considered as a novel antibiotic source.
In this study, an AMP (QUB-2021) was identified in the skin secretion of Odorrana tiannanensis via “shotgun cloning”. Then, the solid phase peptide synthesis (SPPS) and the reverse phase HPLC were used to produce and purify the peptides for the bioactivity assays. Minimum inhibitory concentration (MIC) assays, minimum bactericidal concentration (MBC) assays and an MTT assay, were utilised to evaluate its antibacterial and anticancer cell activities.
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The results showed that QUB-2021 had a antibacterial activity with the MIC values of 8 and 16 μM and MBC values of 16and 32 μM (S. aureus ATCC CRM 6538 and E. coli ATCC CRM 8739). However, it did not exhibit an effective activity against yeast (C. albicans ATCC10231). As for antiproliferative activity, it had a considerable anticancer cell effect at 100 μM with the IC50 value of 62.43 μM. Also, it showed weak haemolysis activity on horse red blood cells with a larger than 256 μM HC50 value.
QUB-2021 thus has potential to become a novel bactericidal or anticancer agent. <br><br><i>Thesis embargoed until 31st December 2027 </i>
Links
Where it is published
- DOI doi.org/10.17034/32633337.v1 ↗
DOI / persistent id · from zivahub uct ac za
Catalogue records · 1
- OAI-PMH record api.figshare.com/v2/oai?verb=GetRecord&metadataPrefix=oai_dc&identifier=oai%3Af… ↗
metadata API · from zivahub uct ac za
Topics
Provenance · 4 source records, 9 field assertions
| Source | Key | Last seen | Raw |
|---|---|---|---|
| ZivaHub | oai:figshare.com:article/32633337 | 9 d ago | JSON v1 |
| Deakin Research Online | oai:figshare.com:article/32633337 | 9 d ago | JSON v1 |
| DMU Figshare | oai:figshare.com:article/32633337 | 9 d ago | JSON v1 |
| UCL Research Data Repository | oai:figshare.com:article/32633337 | 9 d ago | JSON v1 |
| Field | Assertion | Extractor | Evidence |
|---|---|---|---|
| access_level | source · zivahub uct ac za | connector:zivahub_uct_ac_za@1.0.0 | |
| concepts[field].local:field:earth-environmental | mapping · rdr ucl ac uk | connector:rdr_ucl_ac_uk@1.0.0 | |
| concepts[field].local:field:earth-environmental | mapping · zivahub uct ac za | connector:zivahub_uct_ac_za@1.0.0 | |
| concepts[field].local:field:earth-environmental | mapping · dro deakin edu au | connector:dro_deakin_edu_au@1.0.0 | |
| concepts[field].local:field:earth-environmental | mapping · figshare dmu ac uk | connector:figshare_dmu_ac_uk@1.0.0 | |
| description | source · zivahub uct ac za | connector:zivahub_uct_ac_za@1.0.0 | /metadata/dc/description |
| license_text | source · zivahub uct ac za | connector:zivahub_uct_ac_za@1.0.0 | |
| publication_date | source · zivahub uct ac za | connector:zivahub_uct_ac_za@1.0.0 | |
| title | source · zivahub uct ac za | connector:zivahub_uct_ac_za@1.0.0 | /metadata/dc/title |