Text · study · 2026
Aplysia Pleural Ganglia 7 Days After Long-Term Sensitization Training.
Listed in NCBI GEO
We used a custom-designed microarray to characterize the transcriptional response that persists 5 days after long-term sensitization training in the marine mollusk Aplysia californica, a time-point at which recall has partially decayed but there is still clear sensitization memory expressed in most animals.
Description
Aplysia were exposed to a 1-day unilateral LTS training protocol (4 rounds of noxious shock at 30 min intervals, each shock 10s of 1/2s off 1/2on 90mA 60-hz biphasic square-wave shock).
Duration of the tail-elicited siphon withdrawal reflex was measured before training, 1 day after training, and 5 days after training. From the 1-day post-tests we selected 32 animals showing strong sensitization responses (at least 30% increase in reflex duration on the side of training). At the 5-day post-test we observed substantial but not complete decay of the expresison of sensitizaiton.
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Plueral ganglia were harvested immediately after the 5-day post tests for microarray analysis; these ganglia contain the VC nociceptors known to help encode sensitization memory. Samples from a left-trained and right-trained animal were combined to smooth out lateralized gene expression (32 animals produced 16 microarray samples, each having a set of trained plueral ganglia and a set of untrained plueral ganglia). In addition, within each batch of animals collected we matched the highest-forgetting left-trained animal with the highest-forgetting right-trained animal, and so on, producing samples consistent in levels of forgetting and considerable diversity in forgetting across samples.
Links
Get the data
- GEO FTP directory ftp.ncbi.nlm.nih.gov/geo/series/GSE315nnn/GSE315391 ↗
download · from NCBI GEO
Where it is published
- GEO accession page ncbi.nlm.nih.gov/geo/query/acc.cgi?acc=GSE315391 ↗
landing page · from NCBI GEO
Documentation and papers
- PRJNA1396645 ncbi.nlm.nih.gov/bioproject/PRJNA1396645 ↗
project · from NCBI GEO
- PubMed 41802864 pubmed.ncbi.nlm.nih.gov/41802864 ↗
publication · from NCBI GEO
Topics
- Stated by source
- Expression profiling by array
- From keywords
- Life Sciences
Provenance · 1 source records, 6 field assertions
| Source | Key | Last seen | Raw |
|---|---|---|---|
| NCBI GEO | GSE315391 | 12 d ago | JSON v1 |
| Field | Assertion | Extractor | Evidence |
|---|---|---|---|
| access_level | source · NCBI GEO | connector:ncbi_geo@1.0.0 | |
| concepts[field].local:field:life-sciences | mapping · NCBI GEO | connector:ncbi_geo@1.0.0 | |
| concepts[method].geo_series_type:expression-profiling-by-array | source · NCBI GEO | connector:ncbi_geo@1.0.0 | /gdstype |
| description | source · NCBI GEO | connector:ncbi_geo@1.0.0 | /summary |
| publication_date | source · NCBI GEO | connector:ncbi_geo@1.0.0 | |
| title | source · NCBI GEO | connector:ncbi_geo@1.0.0 | /title |