Molecular characterization of Aspergillus flavus strains isolated from animal feeds

It is the most prevalent and producer of AFB1, a potent mutagen and carcinogen in humans and animals. The recent molecular and phenotypic data demonstrated existence of different genetic lineages within this species.

In this study, we used a combination of morphological and molecular methods to characterize isolated fungal specimens. Figures and tables: 1-Phenotypic characterization of A. flavus isolates grown on A flavus/parasiticus agar (AFPA) medium. 2-Analysis of PCR products of the five Aspergillus flavus isolates. 3-PCR/RFLP analysis of fungal amplicons. 4-Aspergillus flavus isolates identity from NBCI blast. 5-Genbank accession numbers of the Aspergillus flavus isolates. 6-Alignment of aflR-aflS(J) intergenic spacer region sequences in A. flavus isolates and reference strains. 7-Maximum Likelihood tree showing the phylogenetic relationships between the examined Aspergillus flavus strains and reference strains, based on aflR/aflS(J) intergenic sequence.