The Extract of Periplaneta Americana (L.) Promotes Hair Regrowth in Mice with Alopecia by Regulating the FOXO/PI3K/AKT Signaling Pathway and Skin Microbiota

(2)

Methods

A strategy combining network pharmacology prediction and in vivo experiments was adopted. The efficacy of Periplaneta americana extract was validated by evaluating hair regrowth status and skin pathological staining in C57BL/6J mice. Transcriptomics, metabolomics, RT-qPCR, and 16s rRNA techniques were integrated to dissect the underlying mechanisms of its hair-growth-promoting effects.

(3) Results: PA-011 significantly promoted hair regeneration in depilated mice via multiple mechanisms: Enhanced skin superoxide dismutase activity and upregulated vascular endothelial growth factor expression; Modulated FOXO/PI3K/AKT signaling pathway and restored skin microbiota homeostasis; Accelerated transition of hair follicles from telogen to anagen phase. (4) Conclusion: PA-011 exerts hair-promoting effects through synergistic modulation of FOXO/PI3K/AKT signaling and skin microbiome.

As a novel therapeutic candidate, it warrants further systematic investigation for clinical translation.