Effect of lysis intermixing on the predicted maturation order

JOURNAL: CELL. Article Type: Research Article Authors: Evgeny Onischenko*, Elad Noor*, Jonas S. Fischer*, Ludovic Gillet, Matthias Wojtynek, Pascal Vallotton, Karsten Weis *Equally Contributing Authors Corresponding Authors: Evgeny Onischenko and Karsten Weis Related to Figure S5 Possible maturation orders in NPC biogenesis, are represented by a binary trees whose leaves are the 9 identified NUP assembly groups.

The leafs are allowed to gradually merge representing the co-assembly events among NUPs until they form a complete NPC (tree root). Each leaf and each vertex (i.e. a mature complex of several such leaves) in the tree is assigned a single variable which represents its relative pool size. For every possible tree configuration this set of variables is optimised to match the effective maturation pool data from the KSM fitting.