<p>Biochemical validation of interaction region between Vpr and TET2.</p>

(<b>B-C</b>) The TET2 disordered loop is essential for Vpr binding. The SEC elution profiles (top) and the corresponding SDS-PAGE analysis (bottom) are shown for (<b>B</b>) NusA-Vpr incubated with the TET2 catalytic domain lacking the internal loop (residues 1129–1936, Δ1481–1843) and (<b>C</b>) NusA-Vpr incubated with the intact TET2 catalytic domain (residues 1129–1936).

The co-elution of Vpr and TET2 observed in (<b>C</b>) and the absence of co-elution in (<b>B</b>) demonstrates that the TET2 internal disordered loop is essential for the formation of a stable Vpr-TET2 complex. Asterisk indicates a contaminating protein.</p> <p>(TIF)</p>