Electrospun Nanofibers for the Delivery of Endolysin/Dendronized Ag-NPs Complex Against Pseudomonas aeruginosa

This work presents the use of electrospun PLCL/PVP (poly(L-lactide-co-ϵ-caprolactone/polyvinylpyrrolidone) nanofibers modified with two agents with antibacterial properties but with different mechanisms of action, that is, dendritic silver nanoparticles (Dend-AgNPs) and endolysin. Results: The nanomat prepared in this manner showed significant antibacterial activity against antibiotic-resistant Pseudomonas aeruginosa strains, inhibiting their growth and production of key pigments and virulence factors.

Moreover, the use of nanofibers as carriers of the selected factors significantly reduced their cytotoxicity towards human fibroblasts. Conclusion: The results confirmed the possibility of using the presented product as an innovative dressing material, opening new perspectives for the treatment of wounds and combating bacterial infections with drug-resistant bacteria.