Impact of Different Nitrogen Fertilizers on the Diversity and Abundance of Bacterial and Fungal Communities in Grassland Soils: The Emerging Case of Ammonium Sulfate and Sulfammox Process [* Cross-Reference *]

Biogas residues (BRs) being a relatively new biofertilizer aiming at substituting chemical nitrogen fertilizers, have raised questions regarding their biosecurity and environmental footprint. In this study, we explored and compared the effect of repeated application of different nitrogen fertilizers on the bacterial and fungal α-diversity, relative abundance, β-diversity, and taxonomic composition in grassland soils over a period of two years.

Given the paramount importance of arbuscular mycorrhizal fungi in sustainable agriculture and climate change, we examined the relative abundance of Glomeromycota and their response to the different nitrogen fertilizers. Finally, the soil microbial community was scanned for the most prominent pathogens that are often detected in BRs and are the main concern related to their application on agricultural soils. Microbial communities in the soil were identified and quantified via high-throughput sequencing of the 16S rDNA marker gene for bacteria, and the ribosomal DNA Internal Transcribed Spacer (ITS2) region for fungi.