Lipid Acyl Chain-Driven α-Synuclein Fibril Polymorphisms and Neuronal Pathologies

Solid-state NMR spectra and microscopy images associated with the following publication: Y. Baek, A. Alim, Y. Dong, T. Olabi, J. Paek, and M. Lee, “Lipid Acyl Chain-Driven α-Synuclein Fibril Polymorphisms and Neuronal Pathologies,” ACS Chemical Neuroscience 17 (7), 1286–1302 (2026). doi.org/10.1021/acschemneuro.5c00730. lipid free_Aged_Neuron_asyn ssNMR: Agedmem_2DCC.ucsf: 2D CC ssNMR spectra of a-syn fibrils formed in the presence of Aged mimetic neuronal membranes Agedmem_2DNC.ucsf: 2D NC ssNMR spectra of a-syn fibrils formed in the presence of Aged mimetic neuronal membrane Neuronmem_2DCC.ucsf: 2D CC ssNMR spectra of a-syn fibrils formed in the presence of normal mimetic neuronal membranes Neuronmem_2DNC.ucsf: 2D NC ssNMR spectra of a-syn fibrils formed in the presence of normal mimetic neuronal membrane lipid_free_2DCC.ucsf: 2D CC ssNMR spectra of a-syn fibrils formed without lipids lipid_free_2DNC.ucsf: 2D NC ssNMR spectra of a-syn fibrils formed without lipids Microscope images: NF-kB: nuclear translocation of NF-κB α-syn aggregation: Immunofluorescence micrographs showing differential formation of intraneuronal α-syn aggregates in response to structurally distinct α-syn PFFs and lipid conditions. pSer 129: Immunostaining of α-syn phosphorylated at serine 129 Supporting information: Differentiation of neuroblastoma cells into dopaminergic neurons