Table 1_Screening for medicine and food homologous ingredients targeting ALDH2 to alleviate acute ethanol-induced liver injury.docx

Aldehyde dehydrogenase 2 (ALDH2) plays a critical role in alcohol metabolism, and ALDH2 deficiency exacerbates alcohol-induced hepatic injury.</p>Aim<p>This study aimed to identify and validate natural ALDH2 activators from medicine and food homologous sources for alleviating acute ethanol-induced liver injury.</p>Methods<p>A literature-based screening revealed 18 candidate compounds.

In vitro enzyme kinetics, molecular docking, and isothermal titration calorimetry were employed to identify four potent ALDH2 activators from these 18 candidates. The hepatoprotective effects of the four candidates were validated in vivo using serum biochemical analyses, oxidative stress measurements, and histopathological examinations in the Gao-binge ALD mouse model.</p>Results<p>Puerarin, sophoricoside, quercetin, and daidzin were preliminarily identified as mixed-type ALDH2 activators, which increased the acetaldehyde dehydrogenation rate catalyzed by ALDH2.