Genetic deletion of LOX-1 attenuates metabolic dysfunction-associated steatohepatitis in mice

The PCR reaction system included DNA, primers, 2×Taq premix and ddH2O, and the amplified products were subjected to agarose gel electrophoresis for visualization and image acquisition. Primer sequences for PCR were listed in Supplement Table 1.Supplement table2: Total RNA was extracted from the liver tissues of mice or LX-2 cells using RNAquick Purification Kit (Shanghai Yishan Biotechnology, Shanghai, China) as described by the manufacturer. cDNA synthesis was carried out with HiScript Ⅱ Q Select RT SuperMix for qPCR (Vazyme).

Quantitative PCR was performed in technical triplicates using SYBR Green reagent (Vazyme). The level of β-actin or GAPDH RNA expression was used to normalize the data. Primer sequences for RT-qPCR were listed in Supplement Table 2.Supplement table3:Gene functional annotation of LOX-1-related MASH targets.