Data · dataset · 2026
RNAseq
Listed in ScienceDB
2.3. RNA-sequencing and bioinformatic analysisTotal RNA was isolated from kidneys using Trizol (Thermo Fisher, 15596018).
Description
Samples with RIN > 7.0 (Agilent Bioanalyzer 2100) were used for library construction and sequenced on an Illumina NovaSeq 6000. Reads were mapped to the Mus musculus genome via HISAT2 (v2.2.1) and quantified as fragments per kilobase of transcript per million mapped reads (FPKM).
Differentially expressed genes (DEGs) were identified using DESeq2 or edgeR, applying a threshold of fold change ≥2 and false discovery rate (FDR) < 0.05. Gene Set Enrichment Analysis (GSEA v4.1.0) was performed against GO and KEGG databases. Genes were ranked by the Signal2Noise method, with significant enrichment defined by |NES|>1, NOM p-val<0.05, FDR q-val<0.25.
Links
Where it is published
- DOI doi.org/10.57760/sciencedb.38165 ↗
DOI / persistent id · from scidb cn
Catalogue records · 1
- OAI-PMH record scidb.cn/oai?verb=GetRecord&metadataPrefix=oai_dc&identifier=10.57760%2… ↗
metadata API · from scidb cn
Topics
- From keywords
- Earth & Environmental Science · Engineering · Humanities · Life Sciences · Social Science
- Inferred from text
- Bioinformatics and computational biology 68% · RNA sequencing 65%
Provenance · 1 source records, 12 field assertions
| Source | Key | Last seen | Raw |
|---|---|---|---|
| ScienceDB | 10.57760/sciencedb.38165 | 10 d ago | JSON v1 |
| Field | Assertion | Extractor | Evidence |
|---|---|---|---|
| access_level | source · scidb cn | connector:scidb_cn@1.0.0 | |
| concepts[field].anzsrc:group:3102 | enrichment · scidb cn | taxonomy-embedding@1.0.0 | title+keywords+description (68%) |
| concepts[field].local:field:earth-environmental | mapping · scidb cn | connector:scidb_cn@1.0.0 | |
| concepts[field].local:field:engineering | mapping · scidb cn | connector:scidb_cn@1.0.0 | |
| concepts[field].local:field:humanities | mapping · scidb cn | connector:scidb_cn@1.0.0 | |
| concepts[field].local:field:life-sciences | mapping · scidb cn | connector:scidb_cn@1.0.0 | |
| concepts[field].local:field:social-science | mapping · scidb cn | connector:scidb_cn@1.0.0 | |
| concepts[modality].local:modality:rna-seq | enrichment · scidb cn | keyword-concept-rules@1.0.0 | title+description (65%) |
| description | source · scidb cn | connector:scidb_cn@1.0.0 | /metadata/dc/description |
| license | source · scidb cn | connector:scidb_cn@1.0.0 | /metadata/dc/rights |
| publication_date | source · scidb cn | connector:scidb_cn@1.0.0 | |
| title | source · scidb cn | connector:scidb_cn@1.0.0 | /metadata/dc/title |