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Data · dataset · 2025

Microscopy data set of mRNA FISH and Protein gene expression measurements in which repressor binding strength is systematically altered.

Listed in Researchdata.se

This dataset contains the raw data, analysis and code needed to generate the figures presented in the paper "Anti-correlation of LacI association and dissociation rates observed in living cells" (see Related Material).

Description

The raw data consists of microscopy images and qPCR files. The analysis and code consist of data analysis, output from the analysis, its post-processing, simulations, and which figure the data was used in.

Experimental data, and analysis scripts are all organized using unique IDs (UID; seen for example in the filenames of experimental data below). The README.txt file in the Analysis_of_data folder describes which UIDs for data, analysis, code & figure generation to combine for specific figures. For the microscopy experiments (has the prefix Microscopy_Data_ in their filenames) there is information on the growth condition and which figure the data was used in.

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All experiments were performed at 30 degrees Celsius. Details on the output from microscopy experiments can be found in the file MicroscopyInformation.txt.

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Where it is published

Catalogue records · 1

Topics

Inferred from text
Image 65% · Microscopy 75%
Provenance · 1 source records, 10 field assertions
SourceKeyLast seenRaw
Researchdata.seoai:researchdata.se:doi-10-17044-scilifelab-26425576/010 d agoJSON v1
FieldAssertionExtractorEvidence
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concepts[field].local:field:earth-environmentalmapping · researchdata seconnector:researchdata_se@1.0.0
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concepts[field].local:field:medicine-healthmapping · researchdata seconnector:researchdata_se@1.0.0
concepts[modality].local:modality:imageenrichment · researchdata sekeyword-concept-rules@1.0.0title+description (65%)
concepts[modality].local:modality:microscopyenrichment · researchdata sekeyword-concept-rules@1.0.0title+description (75%)
descriptionsource · researchdata seconnector:researchdata_se@1.0.0/metadata/dc/description
licensesource · researchdata seconnector:researchdata_se@1.0.0/metadata/dc/rights
publication_datesource · researchdata seconnector:researchdata_se@1.0.0
titlesource · researchdata seconnector:researchdata_se@1.0.0/metadata/dc/title