QUB-1904: A Bioactive Peptide from the Frog Skin Secretion of Pelophylax esculentus

BBIs have various functions, such as for suppress cancer cell proliferation and decrease inflammatory responses, etc. Recent developments in the field of BBIs have led to a renewed interest in amphibian skin-derived BBIs which have a much shorter chain and remarkable inhibitory effect. <br><br>In this study, a bioactive peptide, QUB-1904, was discovered from skin secretion of Pelophylax esculentus by shotgun cloning of a novel biosynthetic precursor encoded cDNA smoothly.

Through the bioinformatic analysis, the amino-acid sequences of mature peptide was determined as SAPRGCWTKSYPPKPCK and ended with a C terminal amide. After chemically synthesis, RP-HPLC and MALDI were subjected to the detection and purification of QUB-1904. The lyophilised pure peptide replicates were subjected to different assays and examinations were operated to evaluate different aspects of bioactivity of QUB-1904.