Data · dataset · 2026
The discovery of a novel bioactive peptide identied from the skin secretion of <i>Hylarana spinulosa</i>
Listed in ZivaHub and Deakin Research Online and DMU Figshare — shown once because both records carry DOI 10.17034/32641863.v1
Amphibian skin secretions are rich in antimicrobial peptides (AMPs), vital components of innate immunity.
Description
With increasing antibiotic resistance, AMPs are promising alternatives due to broad activity and unique mechanisms. In this study, a novel AMP of the Brevinin-2 family was identified from the skin secretion of Hylarana spinulosa, a species rarely studied for bioactive compounds. mRNA from skin secretions was used to clone the gene by RACE, and bioinformatics predicted its sequence and structure.
The peptide, QUB-3002, was synthesised by SPPS, purified by HPLC, and confirmed by MALDI TOF MS. QUB-3002 showed strong antimicrobial activity, with MICs of 0.5 μM against S. aureus, 1 μM against E. coli, and 1 μM against C. albicans. It caused minimal haemolysis (HC10 = 16 μM).
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Antiproliferative assays revealed effects on lung cancer cells H838 (IC50 = 2.196 μM), though normal fibroblasts MRC-5 also showed sensitivity (IC50 = 2.538 μM). Structural predictions indicated an -helical conformation, consistent with membrane-targeting activity. This work expands the AMP repertoire of H. spinulosa and suggests therapeutic potential for antimicrobial and anticancer applications.<br><br><i>Thesis is embargoed until 31 December 2030.</i><br>
Links
Where it is published
- DOI doi.org/10.17034/32641863.v1 ↗
DOI / persistent id · from zivahub uct ac za
Catalogue records · 1
- OAI-PMH record api.figshare.com/v2/oai?verb=GetRecord&metadataPrefix=oai_dc&identifier=oai%3Af… ↗
metadata API · from zivahub uct ac za
Topics
- From keywords
- Earth & Environmental Science · Earth & Environmental Science · Earth & Environmental Science
- Inferred from text
- Cancer 75%
Provenance · 3 source records, 9 field assertions
| Source | Key | Last seen | Raw |
|---|---|---|---|
| ZivaHub | oai:figshare.com:article/32641863 | 9 d ago | JSON v1 |
| Deakin Research Online | oai:figshare.com:article/32641863 | 9 d ago | JSON v1 |
| DMU Figshare | oai:figshare.com:article/32641863 | 9 d ago | JSON v1 |
| Field | Assertion | Extractor | Evidence |
|---|---|---|---|
| access_level | source · zivahub uct ac za | connector:zivahub_uct_ac_za@1.0.0 | |
| concepts[disease].local:disease:cancer | enrichment · zivahub uct ac za | keyword-concept-rules@1.0.0 | title+description (75%) |
| concepts[field].local:field:earth-environmental | mapping · zivahub uct ac za | connector:zivahub_uct_ac_za@1.0.0 | |
| concepts[field].local:field:earth-environmental | mapping · dro deakin edu au | connector:dro_deakin_edu_au@1.0.0 | |
| concepts[field].local:field:earth-environmental | mapping · figshare dmu ac uk | connector:figshare_dmu_ac_uk@1.0.0 | |
| description | source · zivahub uct ac za | connector:zivahub_uct_ac_za@1.0.0 | /metadata/dc/description |
| license_text | source · zivahub uct ac za | connector:zivahub_uct_ac_za@1.0.0 | |
| publication_date | source · zivahub uct ac za | connector:zivahub_uct_ac_za@1.0.0 | |
| title | source · zivahub uct ac za | connector:zivahub_uct_ac_za@1.0.0 | /metadata/dc/title |