Multi-gene phylogeny and morphological evidence reveal two novel species and two new records of Acrogenospora (Minutisphaerales, Acrogenosporaceae) from aquatic habitats in mainland China

Freshwater fungi are characterized by their high species diversity and perform vital ecological roles. During our survey of Chinese aquatic microfungal diversity, four fungal strains were isolated from freshwater environments in Jiangxi and Guizhou Provinces, China. Through comprehensive morphological assessment and multi-locus phylogenetic analyses based on combined ITS, LSU, SSU, RPB 2, and TEF 1 - α sequences, two new species, Acrogenospora bambusae and A. lignicola, were introduced.

In addition, two previously described species, A. taiwanica and A. thailandica, originally described from China (Taiwan), and Thailand, respectively, are newly reported from mainland China. This study provides detailed morphological descriptions, photographic illustrations, and phylogenetic placements for each taxonomic novelty.