Dataset for the published paper titled Coiled-coil-mediated phase separation of Spef1 for central-pair microtubule organization and function

This dataset consists of seven independent experimental subfolders and one supplementary document file, covering biochemical interaction assays, macromolecular crystallography, static light scattering, protein phase imaging, intracellular fluorescence imaging, FRAP dynamic fluorescence detection, and in vitro microtubule polymerization experiments, all focusing on the biochemical and biophysical characteristics of SPEF1 full-length protein and its microtubule binding system.1.

Co-precipitation Assay FolderThis folder stores raw data from co-precipitation biochemical interaction experiments. 2. Crystal Diffraction Data and Structure FolderAll files here derive from X-ray macromolecular crystallography of SPEF1 coiled coil protein.3. Static Light Scattering (SLS) Folder4. spef1Full length protein-phase diagram Folder5. 20210524 Full-Length Protein Intracellular Fluorescence Assay FolderDated May 24, 2021, this folder archives confocal laser scanning microscopy data of fluorescently tagged full-length SPEF1 expressed in mammalian cells.6. 20211022-FRAP-RT 37 FolderThis subfolder stores Fluorescence Recovery After Photobleaching (FRAP) dynamic imaging data acquired on October 22, 2021, at a constant room temperature of 37 °C to quantify the diffusion kinetics of SPEF1 condensates. 7. in_vitro_MT_polymerization in spef1 dropletThis single document file records full experimental records of in vitro reconstituted microtubule (MT) polymerization assays with purified SPEF1 protein.