Computational and experimental reference data: Molecular dynamics of the intrinsically disordered protein COR15A – A force field validation on structure and dynamics

In this study, we investigated the ability of 20 MD models to accurately simulate structural and dynamic aspects of COR15A, an IDP just on the verge of folding, with a particular focus on their ability to capture subtle structural differences. We employ a two-step approach: (i) validation of short 200 ns simulations against small-angle X-ray scattering (SAXS) data, and (ii) detailed evaluation of the six best-performing MD models through extended 1.2 μs MD simulations against nuclear magnetic resonance (NMR) data, including a single-point mutant with slightly increased helicity.

Only DES-amber and ff99SBws capture helicity differences between wild-type and mutant, but ff99SBws overestimates helicity. Notably, only DES-amber adequately reproduces the COR15A dynamics, as assessed by NMR relaxation times at two different magnetic field strengths. Among the tested force fields, DES-amber emerges as the best MD model for the simulation of COR15A.

Its application provides insights into its dynamic conformational landscape, albeit not perfectly reproducing all experimental data. Our study highlights the need for rigorous force field validation for IDPs, and identifies remaining discrepancies in need of fur ther force-field development.