RNA seq data for flia and flk1 cells sorted from zebrafish embryos treated with BF170

All samples were amplified to obtain cDNA product by using Smart Seq2 method, and the concentrations of amplified products were measured by Qubit 3.0 Flurometer. For library construction, cDNA samples were firstly fragmented to approximately 350 bp by Bioruptor Sonication System.

The fragments were subjected to end repair, addition of A base and adaptor ligation, and then purified using Beckman A mpure XP beads. The libraries were run on Illumina HiSeq platform by double-end sequencing program. Bioinformatics analysis was performed based on GRCz10.88 zebrafish reference genome.