Experimental study on the effect of triptolide on acute pancreatitis by regulating IL-34/CSF-1R/STAT3 signaling pathway

HE staining was used to evaluate the pathological changes of pancreatic tissue. Western blot, immunohistochemistry and immunofluorescence were used to detect the expressions of IL-34, CSF-1R and p-STAT3 proteins.Results Compared with the control group, the expression of IL-34 protein in pancreatic tissues and AR42J cells in the AP group was significantly up-regulated (P<0.05), while the expression of CSF-1R protein and the phosphorylation level of STAT3 were significantly increased (P<0.05), and the levels of IL-6, TNF-α and IL-34 in serum and supernatant were also significantly increased (P<0.05).

Celastrol treatment significantly inhibited the expression of the above proteins (P<0.05), and the levels of inflammatory factors in serum and cell supernatant were also significantly reduced (P<0.05).Conclusion IL-34 is involved in the occurrence and development of early inflammatory response in AP, and Celastrol alleviates the early inflammatory response in AP by inhibiting IL-34/CSF-1R/STAT3 signaling pathway, thus having a certain protective effect on AP.