Loss of Bone Marrow β1/β2-Adrenergic Receptors Reprograms Host-Microbiota Interactions and Reduces Visceral Adiposity During Short-term High-fat Diet Feeding- Supplementary Table 1

The sympathetic nervous system (SNS), in turn, regulates immune function through beta-adrenergic receptor (ADRB) 1 and 2 signaling. Our previous study showed that mice transplanted with whole bone marrow from ADRB1/2 knockout donors (KD mice) exhibited mild immunosuppression and shifts in gut microbiota composition.

Because these systems are each implicated in diet-induced obesity, we examined how KD mice respond to a two-week high-fat diet (HFD) challenge. KD mice had lower visceral fat mass than WT controls on HFD, whereas the between-group difference in weight gain did not reach statistical significance. These findings were accompanied by Bacteroidetes enrichment, altered fecal and serum lipid profiles, and lower colonic expression of the fatty acid transporter FAT/CD36.