Data · dataset · 2026
Identification and functional assessment of a bioactive peptide QUB-2984 from the skin secretion of the red-eyed tree frog, Agalychnis callidryas
Listed in ZivaHub and Deakin Research Online and DMU Figshare and UCL Research Data Repository — shown once because both records carry DOI 10.17034/32631837.v1
Bioactive peptides secreted from amphibians have been studied broadly for their potency as alternative drugs to treat human diseases.
Description
This thesis is about a caerin-related peptide derived from red-eyed leaf frog, Agalychnis callidryas, which provided ideas for further antibiotic research. Herein, the objectives included the isolation, identification, and bioactivity assessment of this bioactive peptide named QUB-2984.
Agalychnis callidryas skin secretions were collected by stimulating the frogs’ skin. The sequence of QUB-2984 (GMWGTVFKGIKTVAKHLLPHVFSSQQS) was obtained by ‘shotgun’ cloning through constructing a cDNA library with isolated mRNA, and tested by DNA Sanger sequencing. It was synthesised by solid phase peptide synthesis, purified by reversed-phase high-performance liquid chromatography and structurally-confirmed by MALDI-TOF MS.
Read the rest (1 more)
The MIC value against gram-positive bacteria S.aureus was 2μM, gram-negative bacteria E.coli was 2μM, and against the pathogenic yeast C. albicans was 8μM. QUB-2984 showed anticancer potency at the minimum concentration of 10-5M towards human cancer cells. In terms of haemolysis, it caused around 50% haemolytic effect at 64μM. Its haemolytic effect implied the possibility of side-effects towards mammalian cells, but its ability to exert both antibacterial and anticancer biological activity makes this study valuable as a pre-study foundation for designing new antibiotics against cancer or infectious diseases. <br><i><br>Thesis embargoed until 31 December 2026</i>.
Links
Where it is published
- DOI doi.org/10.17034/32631837.v1 ↗
DOI / persistent id · from zivahub uct ac za
Catalogue records · 1
- OAI-PMH record api.figshare.com/v2/oai?verb=GetRecord&metadataPrefix=oai_dc&identifier=oai%3Af… ↗
metadata API · from zivahub uct ac za
Topics
- From keywords
- Earth & Environmental Science · Earth & Environmental Science · Earth & Environmental Science · Earth & Environmental Science · Life Sciences · Life Sciences · Life Sciences · Life Sciences
- Inferred from text
- Cancer 75% · Sequencing 75%
Provenance · 4 source records, 15 field assertions
| Source | Key | Last seen | Raw |
|---|---|---|---|
| ZivaHub | oai:figshare.com:article/32631837 | 9 d ago | JSON v1 |
| Deakin Research Online | oai:figshare.com:article/32631837 | 9 d ago | JSON v1 |
| DMU Figshare | oai:figshare.com:article/32631837 | 9 d ago | JSON v1 |
| UCL Research Data Repository | oai:figshare.com:article/32631837 | 9 d ago | JSON v1 |
| Field | Assertion | Extractor | Evidence |
|---|---|---|---|
| access_level | source · zivahub uct ac za | connector:zivahub_uct_ac_za@1.0.0 | |
| concepts[disease].local:disease:cancer | enrichment · zivahub uct ac za | keyword-concept-rules@1.0.0 | title+description (75%) |
| concepts[field].local:field:earth-environmental | mapping · dro deakin edu au | connector:dro_deakin_edu_au@1.0.0 | |
| concepts[field].local:field:earth-environmental | mapping · zivahub uct ac za | connector:zivahub_uct_ac_za@1.0.0 | |
| concepts[field].local:field:earth-environmental | mapping · figshare dmu ac uk | connector:figshare_dmu_ac_uk@1.0.0 | |
| concepts[field].local:field:earth-environmental | mapping · rdr ucl ac uk | connector:rdr_ucl_ac_uk@1.0.0 | |
| concepts[field].local:field:life-sciences | mapping · rdr ucl ac uk | connector:rdr_ucl_ac_uk@1.0.0 | |
| concepts[field].local:field:life-sciences | mapping · dro deakin edu au | connector:dro_deakin_edu_au@1.0.0 | |
| concepts[field].local:field:life-sciences | mapping · figshare dmu ac uk | connector:figshare_dmu_ac_uk@1.0.0 | |
| concepts[field].local:field:life-sciences | mapping · zivahub uct ac za | connector:zivahub_uct_ac_za@1.0.0 | |
| concepts[modality].local:modality:sequencing | enrichment · zivahub uct ac za | keyword-concept-rules@1.0.0 | title+description (75%) |
| description | source · zivahub uct ac za | connector:zivahub_uct_ac_za@1.0.0 | /metadata/dc/description |
| license_text | source · zivahub uct ac za | connector:zivahub_uct_ac_za@1.0.0 | |
| publication_date | source · zivahub uct ac za | connector:zivahub_uct_ac_za@1.0.0 | |
| title | source · zivahub uct ac za | connector:zivahub_uct_ac_za@1.0.0 | /metadata/dc/title |