Data · dataset · 2026
MALDI-TOF-MS spectra for "Validation of a MALDI intact cell mass spectrometry workflow for routine application in monitoring industrial E. coli fermentations"
Listed in TU Wien Research Data
Context and methodology Research domain: Process Analytical Technologies, applications of MALDI-TOF on monitoring/development of bioprocesses Dataset was obtained via MALDI-TOF-MS (UltrafleXtreme, Bruker) analysis of intact E.coli from fermentation samples.
Description
Technical details Dataset structure: Main dataset with 4 separate fermentations and 11 timepoints each (44 samples). Each sample has 8 technical replicates.
Sample preparation optimization spectra, reference to figures is given in the subfolders name: Fig. 2 A) acetonitrile optimization (30,50,70 % acetonitrile in matrix solvent; 8 technical replicates) B) example spectra from optimized workflow Fig. 5 Freeze-thaw influence experiment. Four fermentation samples were subjected to up to five freeze-twaw (FT) cycles. (8 technical replicates) Suppl.
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Info. Fig. S2.A: matrix:sample ratio spectra (1:1, 1:2, 2:1, 3:2; 8 technical replicates) Fig. S2.B: influence of optical density (OD) on spectra (OD 3.5−12; 8 technical replicates) Fig. S8: sample with spiked product (1 pmol) Intensity matrix generated after processing the MALDI-TOF-MS spectra the spectra are unprocessed FlexAnalysis (Bruker) or other tools ( R , MALDIquant package ) may be needed to inspect the data
Links
Where it is published
- TU Wien Research Data record researchdata.tuwien.ac.at/records/b997c-v1720 ↗
landing page · from researchdata tuwien ac at
- DOI doi.org/10.48436/b997c-v1720 ↗
DOI / persistent id · from researchdata tuwien ac at
Catalogue records · 1
- InvenioRDM API researchdata.tuwien.ac.at/api/records/b997c-v1720 ↗
metadata API · from researchdata tuwien ac at
Topics
- Stated by source
- MALDI · Mass spectrometry · Process Analytical Technologies
- From keywords
- Mass spectrometry
- Inferred from text
- Analytical chemistry 72%
Provenance · 1 source records, 11 field assertions
| Source | Key | Last seen | Raw |
|---|---|---|---|
| TU Wien Research Data | b997c-v1720 | 9 d ago | JSON v1 |
| Field | Assertion | Extractor | Evidence |
|---|---|---|---|
| access_level | source · researchdata tuwien ac at | connector:researchdata_tuwien_ac_at@1.0.0 | |
| concepts[field].anzsrc:group:3401 | enrichment · researchdata tuwien ac at | taxonomy-embedding@1.1.0 | title+keywords+description (72%) |
| concepts[modality].local:modality:mass-spectrometry | mapping · researchdata tuwien ac at | vocabulary-mapper@1.0.0 | keywords['Mass Spectrometry'] |
| concepts[topic].invenio_subject:researchdata_tuwien_ac_at:maldi | source · researchdata tuwien ac at | connector:researchdata_tuwien_ac_at@1.0.0 | |
| concepts[topic].invenio_subject:researchdata_tuwien_ac_at:mass-spectrometry | source · researchdata tuwien ac at | connector:researchdata_tuwien_ac_at@1.0.0 | |
| concepts[topic].invenio_subject:researchdata_tuwien_ac_at:process-analytical-technologies | source · researchdata tuwien ac at | connector:researchdata_tuwien_ac_at@1.0.0 | |
| description | source · researchdata tuwien ac at | connector:researchdata_tuwien_ac_at@1.0.0 | /metadata/description |
| license | source · researchdata tuwien ac at | connector:researchdata_tuwien_ac_at@1.0.0 | |
| publication_date | source · researchdata tuwien ac at | connector:researchdata_tuwien_ac_at@1.0.0 | |
| title | source · researchdata tuwien ac at | connector:researchdata_tuwien_ac_at@1.0.0 | /metadata/title |
| updated_date | source · researchdata tuwien ac at | connector:researchdata_tuwien_ac_at@1.0.0 |