Data · dataset · 2026
<p><i>MARCHF5</i> KO does not activate the IRF3 signaling pathway nor lytic reactivation.</p>
Listed in UCL Research Data Repository
<p>Western blots of proteins involved in KSHV lytic reactivation (K-bZIP) or activation of the IRF3 signaling pathway (MAVS, phospho-IRF3) in A. BC-3 and B. BCBL-1 iCas9 <i>MARCHF5</i> KO cells harvested at Day 6 of the growth curves.
Description
BCBL-1 PEL cells treated with 20 ng/mL 12-O-tetradecanoylphorbol-13-acetate (TPA) and 1 mM sodium butyrate (NaB) for 2 days serve as a positive control for viral reactivation. 293T cells transfected with an expression construct for human STING for 2 days serve as a positive control for phospho-IRF3.
Quantification of Western blots are shown on the right normalized to total protein stain and g<i>AAVS1</i>. Statistical differences were calculated using two-way ANOVA with Tukey’s multiple comparison post-hoc test. Adjusted <i>p</i> values reflect post-hoc comparisons between g<i>AAVS1</i> to either <i>MARCHF5</i> gRNA.
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Error bars, standard error of the mean.</p>
Links
Where it is published
- DOI doi.org/10.1371/journal.ppat.1014665.g005 ↗
DOI / persistent id · from rdr ucl ac uk
Catalogue records · 1
- OAI-PMH record api.figshare.com/v2/oai?verb=GetRecord&metadataPrefix=oai_dc&identifier=oai%3Af… ↗
metadata API · from rdr ucl ac uk
Topics
- From keywords
- Biochemistry and cell biology · Cancer · Earth & Environmental Science · Genetics · Infectious diseases · Life Sciences · Medicine & Health · Virology
Provenance · 1 source records, 13 field assertions
| Source | Key | Last seen | Raw |
|---|---|---|---|
| UCL Research Data Repository | oai:figshare.com:article/34049129 | 11 d ago | JSON v1 |
| Field | Assertion | Extractor | Evidence |
|---|---|---|---|
| access_level | source · rdr ucl ac uk | connector:rdr_ucl_ac_uk@1.0.0 | |
| concepts[disease].local:disease:cancer | mapping · rdr ucl ac uk | vocabulary-mapper@1.0.0 | keywords['Cancer'] |
| concepts[field].anzsrc:field:310706 | mapping · rdr ucl ac uk | vocabulary-mapper@1.0.0 | keywords['Virology'] |
| concepts[field].anzsrc:field:320211 | mapping · rdr ucl ac uk | vocabulary-mapper@1.0.0 | keywords['Infectious Diseases'] |
| concepts[field].anzsrc:group:3101 | mapping · rdr ucl ac uk | vocabulary-mapper@1.0.0 | keywords['Cell Biology'] |
| concepts[field].anzsrc:group:3105 | mapping · rdr ucl ac uk | vocabulary-mapper@1.0.0 | keywords['Genetics'] |
| concepts[field].local:field:earth-environmental | mapping · rdr ucl ac uk | connector:rdr_ucl_ac_uk@1.0.0 | |
| concepts[field].local:field:life-sciences | mapping · rdr ucl ac uk | connector:rdr_ucl_ac_uk@1.0.0 | |
| concepts[field].local:field:medicine-health | mapping · rdr ucl ac uk | connector:rdr_ucl_ac_uk@1.0.0 | |
| description | source · rdr ucl ac uk | connector:rdr_ucl_ac_uk@1.0.0 | /metadata/dc/description |
| license | source · rdr ucl ac uk | connector:rdr_ucl_ac_uk@1.0.0 | /metadata/dc/rights |
| publication_date | source · rdr ucl ac uk | connector:rdr_ucl_ac_uk@1.0.0 | |
| title | source · rdr ucl ac uk | connector:rdr_ucl_ac_uk@1.0.0 | /metadata/dc/title |