Data · dataset · 2025
Chromosome-level genome assembly of the Pallas's cat (Otocolobus manul)
Listed in ScienceDB
This study presents a chromosome-level genome assembly of the Pallas's cat (Otocolobus manul) from a individual in Qinghai, integrating PacBio HiFi long-read sequencing, Illumina short-read sequencing, and Hi-C chromatin interaction mapping.
Description
Muscle tissue samples (Sample ID: FSD0220013820–1A) were flash-frozen in liquid nitrogen, with DNA extraction and quality assessment conducted by Novogene Bioinformatics Technology Co., Ltd.
A total of 134 Gb PacBio HiFi reads were generated on the Sequel II platform, while Illumina libraries (350 bp insert size) and Hi-C libraries (using Mbol digestion) were sequenced with PE150 reads. The genome was assembled using hifasm, polished with Pilon, Racon, Arrow, and NextPolish, and scaffolded into 19 pseudo-chromosomes via HiCUP and ALLHIC, achieving 98.02% anchoring rate. The assembly exhibits high completeness (97.6% BUSCO score) and accuracy (Mercury QV = 50.46).
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Genome annotation identified 41.09% transposable elements and 22,699 protein-coding genes, with functional annotations covering 90.37% of genes. The dataset includes genome sequences, annotation files, and raw sequencing data.
Links
Where it is published
- DOI doi.org/10.57760/sciencedb.29579 ↗
DOI / persistent id · from scidb cn
Catalogue records · 1
- OAI-PMH record scidb.cn/oai?verb=GetRecord&metadataPrefix=oai_dc&identifier=10.57760%2… ↗
metadata API · from scidb cn
Topics
- From keywords
- Earth & Environmental Science · Engineering · Humanities · Life Sciences · Social Science
- Inferred from text
- Genetics 72% · Sequencing 75%
Provenance · 1 source records, 12 field assertions
| Source | Key | Last seen | Raw |
|---|---|---|---|
| ScienceDB | 10.57760/sciencedb.29579 | 11 d ago | JSON v1 |
| Field | Assertion | Extractor | Evidence |
|---|---|---|---|
| access_level | source · scidb cn | connector:scidb_cn@1.0.0 | |
| concepts[field].anzsrc:group:3105 | enrichment · scidb cn | taxonomy-embedding@1.0.0 | title+keywords+description (72%) |
| concepts[field].local:field:earth-environmental | mapping · scidb cn | connector:scidb_cn@1.0.0 | |
| concepts[field].local:field:engineering | mapping · scidb cn | connector:scidb_cn@1.0.0 | |
| concepts[field].local:field:humanities | mapping · scidb cn | connector:scidb_cn@1.0.0 | |
| concepts[field].local:field:life-sciences | mapping · scidb cn | connector:scidb_cn@1.0.0 | |
| concepts[field].local:field:social-science | mapping · scidb cn | connector:scidb_cn@1.0.0 | |
| concepts[modality].local:modality:sequencing | enrichment · scidb cn | keyword-concept-rules@1.0.0 | title+description (75%) |
| description | source · scidb cn | connector:scidb_cn@1.0.0 | /metadata/dc/description |
| license_text | source · scidb cn | connector:scidb_cn@1.0.0 | |
| publication_date | source · scidb cn | connector:scidb_cn@1.0.0 | |
| title | source · scidb cn | connector:scidb_cn@1.0.0 | /metadata/dc/title |