BRD4 maintains chromaffin-cell identity and promotes pheochromocytoma progression through a SMAD2-dependent transcriptional program

This approach allowed genome-wide mapping of BRD4 occupancy. Our CUT&Tag and ChIP analyses revealed that BRD4 directly occupies the regulatory regions of key chromaffin-cell genes, including TH, CHGB, and PHOX2A.

These findings demonstrate that BRD4 acts as a direct transcriptional regulator of genes essential for chromaffin-cell identity and catecholamine biosynthesis, providing a mechanistic basis for its role in maintaining chromaffin-cell function and pheochromocytoma progression.