Supporting data for “Comparative Genomics in the genus <i>Caenorhabditis</i> and the Discovery of a Novel Toxin-Antidote System in <i>C. elegans</i>”

Main and supplementary figures cover genetic mapping, inheritance tests, RNA interference, reporter expression, microscopy, sequence comparisons, regional synteny, phylogenetic relationships, and the characterization of the sept-1/zina-1 toxin-antidote system.

Additional plots summarize chromosome assignment quality, chromosome conservation, repeat composition, chromosome component sizes, and the presence or absence of developmental regulatory genes across Caenorhabditis species. Three true-scale locus plots provide synteny-based evidence for gene absence in selected species. Five Excel workbooks contain the underlying supporting information, including candidate gene annotations and alignment statistics, strain names and genotypes, software versions and links, cloning and genotyping primers, DNA constructs, expression cassettes, and reference and mutant allele sequences.