Data · dataset · 2026
Identification of new host proteins essential for the replication of rhinovirus
Listed in ZivaHub and Deakin Research Online and DMU Figshare and UCL Research Data Repository — shown once because both records carry DOI 10.17034/32632809.v1
Rhinovirus (RV) infection causes the common cold and is a major trigger for acute exacerbations of asthma and chronic obstructive pulmonary disease (COPD).
Description
However, conventional antiviral approaches focused on directly targeting RV proteins have not led to the development of approved antiviral drugs. An alternative strategy is to interfere with cellular proteins that are exploited by RV during viral infection, which is less likely to lead to the emergence of antiviral resistance.
However, current understanding of the host-pathogen interactions of RV is limited.<br><br>During infection, RV non-structural proteins (NSPs) interact with cellular proteins to subvert host cells and facilitate viral replication. Some of these cellular proteins, like GBF1, PI4KIIIβ and SETD3, were previously identified and proven to be good antiviral targets. However, we hypothesised that additional NSP-interacting cellular targets exist which could be exploited for antiviral development.
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To identify such new targets, we infected H1-HeLa cells with RV-A16 and pulled-down NSPs (2C/2BC, 3D/3CD, 3A/3AB and 3C/3CD) and their binding partners by NSP-specific affinity purification. Analysis of the pulled-down proteins by quantitative mass spectrometry (MS) revealed a specific enrichment in over 160 cellular proteins, including GBF1, PI4KIIIB and SETD3. Interestingly, numerous cellular proteins that were not previously shown to be involved in RV replication were enriched in independent pull-down experiments targeting several NSPs.
Using small molecule inhibitors and siRNA knockdown, we show that some of these NSP interactors represent novel host factors that are essential for RV replication. In turn, this highlights their potential as drug targets for the treatment of RV infections.<br><br><i>Thesis embargoed until 31 July 2027</i>. <br>
Links
Where it is published
- DOI doi.org/10.17034/32632809.v1 ↗
DOI / persistent id · from zivahub uct ac za
Catalogue records · 1
- OAI-PMH record api.figshare.com/v2/oai?verb=GetRecord&metadataPrefix=oai_dc&identifier=oai%3Af… ↗
metadata API · from zivahub uct ac za
Topics
- From keywords
- Animal protection · Animal protection · Animal protection · Animal protection · Earth & Environmental Science · Earth & Environmental Science · Earth & Environmental Science · Earth & Environmental Science · Life Sciences · Life Sciences · Life Sciences · Life Sciences · Microbiology · Microbiology · Microbiology · Microbiology
- Inferred from text
- Disease 75% · Mass spectrometry 75%
Provenance · 4 source records, 23 field assertions
| Source | Key | Last seen | Raw |
|---|---|---|---|
| ZivaHub | oai:figshare.com:article/32632809 | 8 d ago | JSON v1 |
| Deakin Research Online | oai:figshare.com:article/32632809 | 8 d ago | JSON v1 |
| DMU Figshare | oai:figshare.com:article/32632809 | 8 d ago | JSON v1 |
| UCL Research Data Repository | oai:figshare.com:article/32632809 | 8 d ago | JSON v1 |
| Field | Assertion | Extractor | Evidence |
|---|---|---|---|
| access_level | source · zivahub uct ac za | connector:zivahub_uct_ac_za@1.0.0 | |
| concepts[disease].local:disease:disease | enrichment · zivahub uct ac za | keyword-concept-rules@1.0.0 | title+description (75%) |
| concepts[field].anzsrc:field:300304 | mapping · zivahub uct ac za | vocabulary-mapper@1.0.0 | keywords['pathogens'] |
| concepts[field].anzsrc:field:300304 | mapping · rdr ucl ac uk | vocabulary-mapper@1.0.0 | keywords['pathogens'] |
| concepts[field].anzsrc:field:300304 | mapping · figshare dmu ac uk | vocabulary-mapper@1.0.0 | keywords['pathogens'] |
| concepts[field].anzsrc:field:300304 | mapping · dro deakin edu au | vocabulary-mapper@1.0.0 | keywords['pathogens'] |
| concepts[field].anzsrc:group:3107 | mapping · dro deakin edu au | vocabulary-mapper@1.0.0 | keywords['Microbiology'] |
| concepts[field].anzsrc:group:3107 | mapping · zivahub uct ac za | vocabulary-mapper@1.0.0 | keywords['Microbiology'] |
| concepts[field].anzsrc:group:3107 | mapping · figshare dmu ac uk | vocabulary-mapper@1.0.0 | keywords['Microbiology'] |
| concepts[field].anzsrc:group:3107 | mapping · rdr ucl ac uk | vocabulary-mapper@1.0.0 | keywords['Microbiology'] |
| concepts[field].local:field:earth-environmental | mapping · dro deakin edu au | connector:dro_deakin_edu_au@1.0.0 | |
| concepts[field].local:field:earth-environmental | mapping · rdr ucl ac uk | connector:rdr_ucl_ac_uk@1.0.0 | |
| concepts[field].local:field:earth-environmental | mapping · zivahub uct ac za | connector:zivahub_uct_ac_za@1.0.0 | |
| concepts[field].local:field:earth-environmental | mapping · figshare dmu ac uk | connector:figshare_dmu_ac_uk@1.0.0 | |
| concepts[field].local:field:life-sciences | mapping · dro deakin edu au | connector:dro_deakin_edu_au@1.0.0 | |
| concepts[field].local:field:life-sciences | mapping · figshare dmu ac uk | connector:figshare_dmu_ac_uk@1.0.0 | |
| concepts[field].local:field:life-sciences | mapping · rdr ucl ac uk | connector:rdr_ucl_ac_uk@1.0.0 | |
| concepts[field].local:field:life-sciences | mapping · zivahub uct ac za | connector:zivahub_uct_ac_za@1.0.0 | |
| concepts[modality].local:modality:mass-spectrometry | enrichment · zivahub uct ac za | keyword-concept-rules@1.0.0 | title+description (75%) |
| description | source · zivahub uct ac za | connector:zivahub_uct_ac_za@1.0.0 | /metadata/dc/description |
| license_text | source · zivahub uct ac za | connector:zivahub_uct_ac_za@1.0.0 | |
| publication_date | source · zivahub uct ac za | connector:zivahub_uct_ac_za@1.0.0 | |
| title | source · zivahub uct ac za | connector:zivahub_uct_ac_za@1.0.0 | /metadata/dc/title |