Salt-Assisted High Hydrostatic Pressure Strategy to Achieve High-Efficiency Non-Destructive Bacterial In-activation for Liquid Collagen

Although HHP treatment alone cannot in-activate E. coli, its bacterial in-activation efficacy can be enhanced by appropriately adjusting the environmental parameters of liquid collagen. The obtained experimental results presented that E. coli, incubated in a 0.9-3.0% sodium chloride solution for 6 to 12 hours and followed by HHP treatment at 300 MPa, could be eliminated, which indicating the great potential of salt-assisted HHP for bacterial in-activation.

Meanwhile, the triple-helical structure characterization of liquid collagen, which was characterized by circular dichroism (CD) spectrometer and differential scanning calorimeter (DSC), could be saved under above same HHP treatment conditions. Finally, the non-destructive bacterial in-activation effect from salt-assisted HHP could also be observed in E. coli-contaminated liquid collagen model. We hope that this work can provide a feasible strategy to achieve high-efficiency non-destructive bacterial in-activation for liquid collagen.